Additional blood samples will be taken at the end to measure plasma fatty acids levels. Blood samples will be collected at 0, 0.5, 1, 2, 3, 4, and 5 min following the injection for the measurement of plasma palmitate concentrations. Palmitate (10 ♜i) will be intravenously injected to assess basal lipid uptake into individual organs. ![]() Lipid metabolism in individual organs in conscious mice ![]() Basal whole body and organ-specific glucose metabolism will be calculated as described in the Hyperinsulinemic-euglycemic clamp study. At the end of experiment, mice will be anesthetized, and tissue samples will be taken for organ-specific levels of 2-deoxyglucose-6-phosphate. Basal glucose uptake in individual organs will be measured using an intravenous injection of 2-deoxyglucose and taking blood samples at 5-min intervals for 30 min to determine plasma 2-deoxyglucose concentrations. ![]() Glucose metabolism in individual organs in conscious miceįollowing overnight fast or fed state (5-hr after food removal), glucose (0.05 ♜i/min) will be intravenously infused for 2 hrs in awake mice to assess basal whole body glucose metabolism.
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